Receptors compete for adaptors found in plasma membrane coated pits.
نویسندگان
چکیده
منابع مشابه
Phosphoinositide–Ap-2 Interactions Required for Targeting to Plasma Membrane Clathrin-Coated Pits
The clathrin-associated AP-2 adaptor protein is a major polyphosphoinositide-binding protein in mammalian cells. A high affinity binding site has previously been localized to the NH(2)-terminal region of the AP-2 alpha subunit (Gaidarov et al. 1996. J. Biol. Chem. 271:20922-20929). Here we used deletion and site- directed mutagenesis to determine that alpha residues 21-80 comprise a discrete fo...
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The molecular mechanisms of clathrin-dependent internalization of epidermal growth factor receptor (EGFR) are not well understood and, in particular, the sequence motifs that mediate EGFR interactions with coated pits have not been mapped. We generated a panel of EGFR mutants and stably expressed these mutants in porcine aortic endothelial (PAE) cells. Interestingly, mutations of tyrosine phosp...
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Targeting and recruitment of the plasma membrane (PM) clathrin-coated vesicle adaptor complexes has been studied using an in vitro system based on permeabilized acceptor cells and donor cytosol. Through the use of species- and/or tissue-specific antibodies, only newly recruited exogenous PM adaptors are visualized. Targeting of PM adaptors can be switched from the plasma membrane to a perinucle...
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Endocytosis via clathrin-coated pits and vesicles, and the subsequent fate of endocytosed proteins and other substances, has been extensively studied in animal cells. In plant cells until recently, research on endocytosis was hindered by (a) the supposition that endocytosis was not energetically possible in turgid plant cells, and (b) the presence of cell walls, which limit access to plant plas...
متن کاملAdaptor and clathrin exchange at the plasma membrane and trans-Golgi network.
We previously demonstrated, using fluorescence recovery after photobleaching, that clathrin in clathrin-coated pits at the plasma membrane exchanges with free clathrin in the cytosol, suggesting that clathrin-coated pits are dynamic structures. We now investigated whether clathrin at the trans-Golgi network as well as the clathrin adaptors AP2 and AP1 in clathrin-coated pits at the plasma membr...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1988
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1988.tb03204.x